Nutrient agar is the most commonly used media for filling petri dishes and growing bacteria. This is the powder form of nutrient agar - add water and sterilize for a general purpose nutrient agar base. This 8 g container makes about 250 ml of nutrient agar—enough to cover 20 standard 90-100 mm petri dishes (not included).
Try This Fun Recipe: *Makes 100 ml of nutrient agar solution - enough to cover 7-9 standard 90-100 mm petri dishes.
- Mix 1 level teaspoon of dehydrated agar with 100 ml (3/8 cup) of room-temperature water.
- Bring to a boil while stirring to ensure the agar is completely dissolved.
- Pour 10-12 ml of hot agar into each petri dish, just covering the bottom.
- Replace the dish top immediately after pouring to prevent contamination.
- Let dishes stand for 1 hour, allowing agar to solidify.
- This agar can be sterilized in a pressure cooker before pouring into the sterile plastic dishes. Use 15 lb. pressure for 15 minutes.
- We provide more information about how to use agar & petri dishes
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