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This is the powder form of nutrient agar-- add water and sterilize for a general purpose nutrient agar base. Nutrient agar is the most commonly used media for filling petri dishes and growing bacteria. This 8 g container makes about 250 ml of nutrient agar—enough to cover 20 standard 90-100 mm petri dishes.
This nutrient agar can be sterilized in a pressure cooker before pouring into sterile plastic dishes. Use 15 lbs pressure for 15 minutes.
Instructions: Mix 1 level teaspoon (approximately 2.2 grams) of dehydrated nutrient agar with 100 ml (3/8 cup) of room-temperature water. Bring to a boil while stirring to ensure the agar is completely dissolved. Pour 10-12 ml hot agar in each petri dish, just covering the bottom. Replace the dish top immediately after pouring to prevent contamination. Let dishes stand 1 hour for agar to solidify. This recipe makes 100 ml of nutrient agar solution, enough to cover 7-9 standard 90-100 mm petri dishes.
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